Figure 2
From: Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa
Interaction of PPP1R2P3 with different PPP1 isoforms. (A), Sequential transformation of yeast AH109 with bait plasmid (pAS2-PPP1CA, pAS2-PPP1CC1, pAS2-PPP1CC2, or pAS2-PPP1CC2end) and the prey plasmid pACT2-PPP1R2P3. pAS2-PPP1CC2end is the unique C-terminal tail of PPP1CC2 produced by alternative splicing of the PPP1CC gene. For negative and positive controls pAS2-1/pACT-2 and pVA3-1/pTD1-1 vectors were used, respectively (B), Overlay assay of PPP1R2 and PPP1R2P3. Commercial PPP1R2 and His-PPP1R2P3 were separated by SDS-PAGE, transferred to nitrocellulose and overlaid with recombinant PPP1CC1 or PPP1CC2, as indicated. Western blotting was performed with the respective specific antibodies.