Figure 5

Co-localization of syndecan-2 and fibronectin on Caco-2 and HCT-116 cells. (A) Caco-2 cells were cultured on Petri dishes in the absence(Ctrl) or (B) presence of fibroblast ECM (Fibro. ECM) for three days. (C) HCT-116 cells were cultured on Petri dishes in the absence (Ctrl) or (D) presence of fibroblast ECM (Fibrob. ECM) for three days. Cells were immunestained with anti-fibronectin (III, green) and anti-syndecan-2 (I, red). The nuclei (II, blue) were stained with DAPI. Immunofluorescent images were captured using confocal microscopy. Co-localization of images (IV, Merge). Scale bar represents 20 μm. The relative fluorescence levels of proteins were determined by densiometric analysis and represented as a percentage of controls (E and F). *p ≤ 0.05 compared to control.