PC expression in podocytes. (A) Western blot analysis for PC expression in HGEC. Total protein was extracted from cells cultured either in the presence of 5 mM (control) (lane 1) or 25 mM (lane 2) glucose. Lane 3 represents PC expression in HGEC:25 mM-to-5 mM/18w. Lanes 4, 5, 6 and 7 represent HGEC:5 mM-to-25 mM/1w, HGEC:5 mM-to-25 mM/2w, HGEC:5 mM-to-25 mM/6w and HGEC:5 mM-to-25 mM/18w respectively. Lanes 8 and 9 represent HGEC:25 mM/6w-to-5 mM/4w and HGEC:25 mM/18w-to-5 mM/4w respectively. Blots were reprobed with anti-tubulin antibody, to verify protein loads, against which the data were normalized. (B) Results were expressed as the mean ± SD of four independent experiments (*p<0.05 vs. HGEC:5 mM, **p<0.01 vs. HGEC:5 mM, #p<0.05 vs. HGEC:25 mM).