Table 1 Primers used for PCR amplification to construct EGFP-Rheb and DsRed-Rheb (5' – 3' direction; restriction sites underlined)
Rheb_EcoRI_FP | TGA ATTC GC CGC AGT CCA AGT CCC GG |
Rheb_SalI_RP | GGG GTC GAC TCA CAT CAC CGA GCA TGA AGA C |
DsRed(m)_AgeI_FP | AAC CGG T CG CCA CCA TGG AC |
DsRed(m)_BspeI_RP | GGT CCG GA C TGG GAG CCG GAG TGG CG |