Exogenous β-catenin signaling induces Wnt pathway and stem cell-related network activities in HONE1 hybrid cells. A. RT-PCR analyses for HONE1, MCH903.1, HONE1 hybrid cells (MCH4.4/4.5/4.6) and human embryonic stem cells H7. B. Immunofluorescence staining shows that β-catenin proteins clearly accumulate in the cellular membrane in most of hybrid cells (MCH4.6). C. Western blot analysis reveals that protein expression of β-catenin, Axin2, Nanog, Oct4 and E-cadherin is up-regulated in HONE1 hybrid cells, but N-cadherin is down-regulated. D. Luciferase assay shows increased Wnt activities in HONE1 hybrid cells. STOP/SFOP values are increased by 70-fold in MCH4.6 cells compared to parental HONE1 cells. E. Immunohistochemical staining shows consistent expression of Wnt target genes in mouse models. In tumor samples, protein expressions of β-catenin, E-cadherin, and Cyclin D1 are strongly up-regulated in tissues derived from two HONE1 hybrid cell lines (MCH4.5-1TS and MCH4.6-1TS), 40x magnification. Bars, 20 μm. F. RT-PCR analysis shows that loss of transferred chromosome fragment containing an exogenous β-catenin copy (MCH4.5-2TS) is associated with concomitant loss of up-regulated expression of endogenous genes.