ADF/cofilin depletion in MTLn3 cells decreases mitotic index, and increases multinucleation and micronuclei formation. MTLn3 cells were stained with DAPI and fluorescent-phalloidin and three mitotic parameters were analyzed: mitosis (A), multinucleation (B) and micronucleation (C). D. Cells were scored as in (A-C) and mitotic index, percentage of multinucleation and micronucleation was calculated. n ≥ 600 cells in each experiment, three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 versus control. Scale bar: 10 μm.