Suppression of ADF or cofilin expression in MTLn3 cells enhances cell migration. A. Cells were serum-starved, seeded on collagen I-precoated filters and then subjected to a migration assay. Cell migration is expressed as percent of control cells. Four independent experiments each performed in triplicate. B. MTLn3 cells were grown into monolayers and wounds were made with a sterile tip. The wound area was measured at 0 hr and 6 h, and migration rate was expressed as μm/h. Three independent experiments each performed in triplicate. C. The migration rate was measured from the cell centroid using kymography, four different regions of each cell were selected and a kymograph created for each region, only one of which is shown here. D. The kymograph was analyzed with a journal which tracks the front and rear membrane positions along the line at each time point and creates an average cell center position (centroid) which is then plotted versus time (frame number), from which a slope was calculated. E. The migration rate [slope (pixel/frame) × (1 μm/number of pixels) × (number of frames/number of min)] was averaged from 10 cells in each treatment, 4 measurements (slopes) per cell, three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 versus control.