Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells

Table 1 MS analysis of 20 (19) differential protein spot species in pHPL and tPRP ^{a) b)}

Ratio ‘pHPL’/‘tPRP’	Spot	Protein name	SwissProt ID	Sequence coverage (%)	Peptides queries matched	Percental match (%)	MOWSE score (> 56; p < 0.05)	MW (Da)	pI
0.62	1	Apolipoprotein A-I	sp\|P02647\|APOA1_HUMAN	80	29	33.7	292	30759	5.56
0.65	2	Apolipoprotein A-I	sp\|P02647\|APOA1_HUMAN	64	23	26.4	205	30759	5.56
0.69	3	Retinol-binding protein 4	sp\|P02753\|RET4_HUMAN	67	11	15.3	114	23337	5.76
0.71	4	Angiotensinogen	sp\|P01019\|ANGT_HUMAN	19	9	14.8	62	53406	5.87
0.71	5	CD5 antigen-like	sp\|O43866\|CD5L_HUMAN	28	8	9	66	39603	5,28
0.74	6	Apolipoprotein A-I	sp\|P02647\|APOA1_HUMAN	59	23	29.5	193	30759	5.56
1.55	7	Vitamin D-binding protein	sp\|P02774\|VTDB_HUMAN	37	15	35.7	147	54526	5.40
1.84	8	Hemopexin	sp\|P02790\|HEMO_HUMAN	25	9	10.1	68	52385	6.55
2.68	9	Fibrinogen gamma chain	sp\|P02679\|FIBG_HUMAN	59	21	25.3	201	52106	5.37
2.77	10	Fibrinogen gamma chain	sp\|P02679\|FIBG_HUMAN	56	21	23.1	226	52106	5.37
2.98	11	Tropomyosin alpha-4 chain	sp\|P67936\|TPM4_HUMAN	33	10	17.2	83	28619	4.67
3.35	12	Fibrinogen gamma chain	sp\|P02679\|FIBG_HUMAN	27	13	15.1	88	52106	5.37
4.30	13	Tubulin beta chain	sp\|P07437\|TBB5_HUMAN	39	17	20.7	149	50095	4.78
4.59	14	Fibrinogen gamma chain	sp\|P02679\|FIBG_HUMAN	56	21	24.1	235	52106	5.37
6.00	15	Fibrinogen gamma chain	sp\|P02679\|FIBG_HUMAN	51	19	22.1	189	52106	5.37
6.69	16	Fibrinogen gamma chain	sp\|P02679\|FIBG_HUMAN	27	11	16.7	73	52106	5.37
10.36	17	Chloride intracellular channel protein 1	sp\|O00299\|CLIC1_HUMAN	43	9	18	88	27248	5.09
10.76	18	Actin, cytoplasmic 1	sp\|P60709\|ACTB_HUMAN	53	16	22.2	134	42052	5.29
11.01	19^b)	Annexin A5	sp\|P08758\|ANXA5_HUMAN	21	5	10.4	53^b)	35971	4.94
12.27	20	Actin, cytoplasmic 1	sp\|P60709\|ACTB_HUMAN	52	18	27.3	163	42052	5.29

^a)Mascot software analysis by using SwissProt data base. Ratio pHPL/tPRP: <1 higher abundant in tPRP, >1 higher abundant in pHPL. PMF criteria: human source, one missed tryptic cleavage, methionine oxidation, score limit >56. ^b)Despite the fact that potential MS identification of Annexin A5 is below required scoring (5.6% less), this non-significant MS hit was included into the list because the suggested high ratio and protein ID was confirmed by Western blotting.

ISSN: 2661-8850