Figure 9

Impact of the knockdown of the α6 integrin subunit on the survival of human IECs. A. Representative (n ≥ 3) WB analyses of α6 and actin from adhering HIEC cell cultures, following their infection by a lentivirus carrying GFP (control), shCNS or shα6. Specific antibodies for α6 and actin were used. B. Adhering HIEC cell cultures were infected as in ( A ), except that ISEL assays were performed and compared to controls. C. Adhering HIEC cell cultures were infected as in ( A ), except that CASP-3 relative activity was established, by comparison to controls. A-C. Results obtained with -2PC Caco-2/15 cells were highly similar to those shown here for HIEC cells. B-C. Statistically significant (0.0001 ≤ P ≤ 0.001; n ≥ 3) differences between treated and control cultures are indicated by (*). Statistically significant (0.0005 ≤ P ≤ 0.005; n ≥ 3) differences between shα6 and shCNS are indicated by (#).