Newborn pig trachea cell line cultured in air-liquid interface conditions allows a partial in vitro representation of the porcine upper airway tissue

Table 3 Primer sequences, annealing temperatures of primer sets (°C), expected PCR fragment sizes (bp) and accession numbers or references

Primer name	Primers sequence	Annealing temperature (°C)	PCR product (bp)	Accession number or reference
*ACTB*	CACGCCATCCTGCGTCTGGA AGCACCGTGTTGGCGTAGAG	63	100	[24]
*B2MI*	CAAGATAGTTAAGTGGGATCGAGAC TGGTAACATCAATACGATTTCTGA	58	161	[24]
*GAPDH*	CTTCACGACCATGGAGAAGG CCAAGCAGTTGGTGGTACAG	63	170	AF017079
*HMBS-2*	AGGATGGGCAACTCTACCTG GATGGTGGCCTGCATAGTCT	58	83	[24]
*HPRT-1*	GGACTTGAATCATGTTTGTG CAGATGTTTCCAAACTCAAC	60	91	[24]
KRT 8	TGACCGACGAGATCAACTTC TGATGTTCCGGTTCATCTCC	60	294	NM_001159615
MUC1	TAAAGAAGACGGGCTTCTGG CCGCTTTAAGCCGATCAAAC	60	134	XM_001926883
MUC2	ACCCGCACTACGTCACCTTC GGCAGGACACCTGGTCATTG	62	150	BX671371
MUC4	CTGCTCTTGGGCACTATATG CCTGTGACTGCAGAATCAAC	60	133	DQ848681
OCLN	CTACATAATGGGCGTCAACC GGGCTGCTCGTCATAAATAC	60	298	NM_001163647
*RPL-19*	AACTCCCGTCAGCAGATCC AGTACCCTTCCGCTTACCG	60	147	[25]
*SDHA*	CTACAAGGGGCAGGTTCTGA AAGACAACGAGGTCCAGGAG	58	141	[24]
*TBP-1*	AACAGTTCAGTAGTTATGAGCCAGA AGATGTTCTCAAACGCTTCG	60	153	[24]
VIL1	AGAAGTGGACGGTGCCCAAC TCTCGCCGATGAGGTAGGTG	64	273	XM_001925167
ZO-1/TJP1	GAGGGCATTTCCCACGTTTC GCTTTAGAGCCGAGTCCTTG	62	256	XM_003353439

ISSN: 2661-8850