Figure 3

Loss of integrity of cell membrane of HUVEC after exposure to neutrophil MPs and H ₂ O ₂ . CFDA-labeled cells (A, D, G, J, M and P); PI-labeled cells (B, E, H, K, N and Q); merged figures (C, F, I, L, O and R). Control HUVECs incubated with medium alone (A-C). HUVECs incubated with 5 μg/mL MPs (D-F). HUVECs incubated with 20 μM H₂O₂(G-I). HUVECs incubated with 5 μg/mL MPs and 20 μM H₂O₂(J-L). HUVECs incubated with 5 μg/mL MPs, 20 μM H₂O₂ and 10 μM sodium azide (M-O). HUVECs incubated with 5 μg/mL MPs, 20 μM H₂O₂ and 1 μg/mL MPO inhibitor-I (P-R). Magnification: 400×. The results shown in each panel are representative of four independent experiments.