Figure 4

Neutrophil MPs induce cell injury by MPO activity. HUVEC cells were incubed with neutrophil MPs (5 μg/mL) in the presence or absence of 20 μM H₂O₂, 10 μM sodium azide and 1 mM taurine, for 30 min in 5% CO₂ at 37°C. After the treatments, the cells were submitted to AnV and PI labeling and analyzed by flow cytometry. The injury caused by the MPO system (MPs and H₂O₂) and not the injury induced by H₂O₂ alone was decreased in the presence of sodium azide or taurine, inhibitors of the MPO pathway. The values represent the percent of AnV and PI positives cells in each treatment in relation to the total cells analyzed.