Figure 7

GPR40 activation improves insulin synthesis and secretion. (A) After chronic culture for 72 h, rat islets were pre-treated with low glucose containing KRBH followed by treatment for 2 h with low or high glucose. Amount of secreted insulin in buffer (A) and intracellular insulin content (D) were measured using ELISA. Likewise, NIT1 cells were also cultured and treated followed by cellular ATP levels estimation using luciferase based luminescence assay (B). Insulin gene transcripts (C) were measured in rat islets after chronic culture under indicated condition by qRT-PCR. (n = 4, *P < 0.05, **P < 0.01, ***P < 0.001).