Figure 3

Cytological evaluation of BMCs using Giemsa stain. Treated cells were incubated for 96 hours. a) Untreated cells. b) Cells treated with 100 μg/mL AEPa, note the increased cell spreading, cytoplasmic volume and cells with characteristics of activated macrophages (arrow). c) Cells treated with 100 nM of M-CSF. Cells after treatment with M-CSF presented a significant spreading ability, increased cellular area and most of the cells are undergoing cell fusion processes (head arrows). Scale bar 10 μm. d) Morphometric analysis showed increased cell area for cells treated with AEPa or M-CSF, compared with control cells. Data are presented as means ± SD, p < 0.05.