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Table 1 Phenotypes of transfected cell lines expressing inducible GFP-BLM alleles.

From: The C-terminal domain of the Bloom syndrome DNA helicase is essential for genomic stability

    Localization Relative Relative
Cell Line % green cells % green of GFP-BLM Expression Expression
  no inducer cells +Tet fusion proteins no inducer +Tet
GFP 8 33 diffuse (NM1) nd nd
GFP-BLM 1 94 NBs + NM2 0.16(0.03) 1.0(0.15)
GFP-ΔN1 1 75 NM2 (3% NBs) 0.17(0.05) 0.96 (0.3)
GFP-ΔN2 9 90 NM2 0.30(0.08) 2.28 (0.35)
GFP-ΔN3 2 74 NM2 + BBs 0.05(0.02) 0.87(0.12)
GFP-ΔN4 1 77 NM1&2 0.09(0.01) 0.81 (0.09)
GFP-K695T 3 30 NBs + NM2 + BBs 0.12(0.01) 0.45 (0.08)
GFP-ΔH1 3 80 NBs +NM3 0.02(0.01) 0.39 (0.08)
GFP-ΔC1 1 77 NBs + BBs 0.04(0) 0.83 (0.03)
GFP-ΔC2 5 82 NBs + BBs 0.01(0) 0.74 (0.06)
  1. At least 200 DAPI-stained cells were counted and scored for green fluorescence to determine the percentages of induced and uninduced cells expressing the GFP-BLM fusion proteins. All GFP-fusion proteins show diffuse nuclear staining. The nucleolar morphologies (NM) of each fusion protein are reported as shown in Figure 4. NM1 is a diffuse overall nucleolar localization (Figure 4A &4H). NM2 is overall nucleolar fluorescence with exclusion from central holes " TOPIIIα. BBs are numerous spherical BLM-containing nuclear bodies that do not co-localize with either antigen. At least 300 cells were examined for determination of nuclear morphologies. Expression levels of the fusion proteins without Tet and with 1 μg/ml Tet relative to normal BLM are reported as determined in Figures 3 &5. The values reported are the average of two experiments. The standard deviation for these data points is shown in parentheses beside each value.