Effect of an AGE-modified type I collagen matrix on MC3T3E1 cell growth. MC3T3E1 calvaria-derived osteoblastic cells were cultured on control collagen or AGE-modified collagen at 37°C for one week (differentiated osteoblasts) or three weeks (mineralizing cultures). The quantity of cells growing on the corresponding collagenous matrices at each timepoint, was evaluated by trypsinization and cell counting with a haemocytometer. Results are given as the number of cells counted in each case, and are expressed as the mean ± SEM. Differences between collagen vs. AGE-collagen are as follows: ** p<0.001.