Figure 4

Cargo domains repeatedly shed post-Golgi carriers Transiently expressed VSVG3-YFP cargo was accumulated in the ER and pulsed into the secretory pathway of PtK₂ cells as in Figure 1B. Time at 32°C and the frame number of the corresponding movie (Additional Fileset 4 are indicated. Cargo domains develop on Golgi elements and post-Golgi TCs repeatedly exit from these domains. discrete exit events are numbered. Event (4) shows coalescence of a large cargo domain prior to the exit event (5). During exit event (8), the TC extends the domain as if the TC and domain were still connected (9). It is especially apparent in the movie (Additional Fileset 4) that cargo domains and regions of Golgi elements move as if they were connected. Bar: 5μm.