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Table 2 Changes of mitochondrial membrane potential, assessed by flow cytometry using JC-1 red/green fluorescence ratio in in control and Scott cells after 48 or 72 h of apoptosis induction by calcium ionophore A23187 (200 nM) or SKF 96365 (10 μM) in the absence and presence of the caspase inhibitor z-VAD.fmk (50 μM). Values are the mean ± SEM of 4 independent experiments.

From: Deciphering the plasma membrane hallmarks of apoptotic cells: Phosphatidylserine transverse redistribution and calcium entry

CELLS No treatment A23187 (200 nM) SKF 96365 (10 μM) zVAD + A23187 zVAD + SKF 96365
Control (48 h) 1.37 ± 0.24 1.05 ± 0.16 0.85 ± 0.11* 1.16 ± 0.03 1.01 ± 0.09
Scott (48 h) 1.25 ± 0.20 1.01 ± 0.17 0.96 ± 0.20* 1.11 ± 0.08 1.20 ± 0.12
Control (72 h) 1.26 ± 0.09 1.1± 0.16 0.89 ± 0.06* 0.97 ± 0.03 0.88 ± 0.04*
Scott (72 h) 1.55 ± 0.12 1.02 ± 0.22* 1.04 ± 0.26* 0.97 ± 0.16* 0.89 ± 0.21*
  1. *P < 0.05 versus the corresponding values obtained with non-treated cells.