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Table 1 Effect of neomycin sulfate and propranolol on the L-T4-induced DAG formation in [14]oleic acid pre-labeled hepatocytes.

From: Thyroxine signal transduction in liver cells involves phospholipase C and phospholipase D activation. Genomic independent action of thyroid hormone

Time, Additions Control L-thyroxine % of control
min     
   % total14C-lipids  
  NaOH 0.96 ± 0.11 1.34 ± 0.09* 140
0.25 NaOH+neomycin sulfate 1.43 ± 0.37 1.38 ± 0.04 96.7
  NaOH 1.19 ± 0.06 2.02 ± 0.14* 170
2.0 NaOH+neomycin sulfate 1.08 ± 0.11 1.43 ± 0.09 132
  NaOH+ propranolol 1.97 ± 0.21 1.77 ± 0.15 90
  1. [14C]oleic acid labeled hepatocytes were pre-incubated for 15 min prior to NaOH (l00 nM) or LT4 (l0 nM) addition or pre-incubated for 15 min with neomycin sulfate (1.5 mM) or propranolol (100 nM) prior to stimulation with L-T4 (l0 nM).The data are mean ± S.E. of four separate experiments. * P < 0.05 vs. control.