Thyroxine signal transduction in liver cells involves phospholipase C and phospholipase D activation. Genomic independent action of thyroid hormone

Table 1 Effect of neomycin sulfate and propranolol on the L-T₄-induced DAG formation in [¹⁴]oleic acid pre-labeled hepatocytes.

Time,	Additions	Control	L-thyroxine	% of control
min
		% total¹⁴C-lipids
	NaOH	0.96 ± 0.11	1.34 ± 0.09^*	140
0.25	NaOH+neomycin sulfate	1.43 ± 0.37	1.38 ± 0.04	96.7
	NaOH	1.19 ± 0.06	2.02 ± 0.14^*	170
2.0	NaOH+neomycin sulfate	1.08 ± 0.11	1.43 ± 0.09	132
	NaOH+ propranolol	1.97 ± 0.21	1.77 ± 0.15	90

[¹⁴C]oleic acid labeled hepatocytes were pre-incubated for 15 min prior to NaOH (l00 nM) or LT₄ (l0 nM) addition or pre-incubated for 15 min with neomycin sulfate (1.5 mM) or propranolol (100 nM) prior to stimulation with L-T₄ (l0 nM).The data are mean ± S.E. of four separate experiments. ^* P < 0.05 vs. control.

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