Figure 2
From: Cell density related gene expression: SV40 large T antigen levels in immortalized astrocyte lines
Western blot of Tag expressing cell lines. (A) Cell extracts of each line and a purified baculovirus lysate of recombinant Tag were Western blotted (see Materials and Methods). The P0-13D tkT#13 lane contained lysate from 2.5 × 103 cells; for the other lines, lysates from 1.25 × 103 cells were loaded. NIH 3T3 extract was added to the low protein extracts and the authentic Tag so that total protein was equal in each lane. (B) Densitometry of the Western blot was compared to total Tag immunofluorescence determined by cytometry for each cell line.