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Table 2 Effect of serum on Tag and %G11.

From: Cell density related gene expression: SV40 large T antigen levels in immortalized astrocyte lines

Serum (%)

Cells2 (×106)

T Antigen

%G1

2.5

3.99

60.4

71.1

5

4.02

57.6

73.7

10

3.62

57.1

71.8

  1. 1 The P0-3DtkT#5 cell line was inoculated at 106 per 10 cm plate in DMEM containing the indicated amount of serum. Cells were harvested after 3 days and analysed for Tag level and %G1 by cytometry. 2Cell number = final cell number per 10 cm dish.