Figure 4

Levels of FN in the granulation tissue of excision wounds. Paraffin-embedded sections of excision wounds treated every other day with vehicle or 1 μg cCAF were immunostained with an antibody to FN. Three days after wounding, control wounds (A) show less labeling for FN than those treated with cCAF (B). (C) Immunoblot analysis of protein extracted from treated and control wounds reveals that treatment with cCAF causes increased FN levels at 3 days but at 7 days the FN protein levels to approximately the levels of the control.