Figure 2
Effects of different phospholipids on the proteolysis of Boi1-PH. Shown are immunoblots, probed with anti-Boi1 antibodies, of aliquots from a Boi1-PH-bearing yeast extract that was incubated, in the presence of 2 mM CaCl2 (to allow proteolysis), with vesicles containing PS (part A) or PC (part B). The vesicles contained either no additional lipid (lanes 1) or a 1:20 (mass:mass) ratio (with respect to PS or PC) of PI (lanes 2), PI3P (lanes 3), PI4P (lanes 4), or PIP2 (lanes 5).