TIRF images of GFP-myosin II (A) and GFP-MHCK-C expressed in the presence of myosin II (B). The fluorescent images show GFP-myosin II thick filaments and GFP-MHCK-C particles in the cortex of a cell attached on a coverslip with a refractive index of 1.78. The distribution of the rod length is displayed next to the images. The mean length of GFP-myosin II and GFP-MHCK-C is 0.6 and 0.3 μm, respectively. The scale bar is 3 μm.