Figure 7
From: Phorbol ester induces elevated oxidative activity and alkalization in a subset of lysosomes
The effect of H 2 O 2 and O 2 - on the fluorescence of LysoTracker Red DND-99. 2 μM of LysoTracker Red DND-99 was prepared in MES buffer solutions at either pH 4.5 (panel A) or pH 7.5 (panel B). H2O2 (◆) or KO2 (■) were then added to various final concentrations and the resulting reaction solutions were incubated at room temperature for 10 min. Fluorescence of the LysoTracker Red was then determined.