Figure 8

Localization of Rab24 deletion mutants and Rab24/Rab1B chimeras. 293 cells were transfected with expression vectors encoding (A,B) Rab24wt or Rab24(D123I), with deletions in the loop-8 insert region, or (C-F) a series of chimeric proteins composed of combinations of the N-terminal and C-terminal domains of Rab24 and Rab1B, with or without the D123I substitution (see Methods for detailed descriptions). After 24 h, the transfected cells were processed for immunofluorescence microscopy using the anti-myc monoclonal antibody and FITC-conjugated GAM IgG to localize the expressed proteins. Panels A-F show representative cells from cultures expressing the indicated proteins. The bar represents 10 microns.