Figure 4
Expression of transgenic Rab27a proteins in mouse tissues. Protein extracts obtained from eyes, spleen, liver, skin, lung, kidney, stomach, large intestine, small intestine, brain, testis and heart (50 μg each) were subjected to SDS-PAGE and probed with monoclonal anti-myc-tag antibody (9E10) and/or a specific anti-Rab27a monoclonal antibody (4B12). Calnexin was used as a loading control and detected using a specific polyclonal antibody. A protein extract of HeLa cells transfected with myc-Rab27aT23N was used as a positive control. (Panel A) Tissues from the A27aT25/2 line carrying Rab27aT23N. (Panel B) Tissues from the A27aQ24 line carrying Rab27aQ78L.