Skip to main content

Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Table 1 Quantitation of myosin-II distribution during cytokinesis : Comparison with minimal force requirements.

From: Quantitation of the distribution and flux of myosin-II during cytokinesis

  Interphase Spherical Cylindrical Dumbbell
Cell volume, μm3 a 620 620 690 720
Volume of cell cortex, μm3 a 120 120 130 160
Volume of furrow, μm3 a - - 40 20
Total wild type myosin-II, % total [μM] 100 [3.4] 100 [3.4] 100 [3.4] 100 [3.4]
   Cytoplasmb 77 ± 2 [3.2] 79 ± 2 [3.4] 79 ± 2 [3.4] 73 ± 2 [3.2]
   Total cortexb 23 ± 0.3 [4.0] 21 ± 0.5 [3.6] 21 ± 0.5 [3.8] 27 ± 0.5 [4.2]
   Furrow cortexb - - 8.4 ± 0.2 [5.0] 5.3 ± 0.4 [6.4]
Total 3 × Ala myosin-II, % total [μM] 100 [3.4] 100 [3.4] 100 [3.4] 100 [3.4]
   Cytoplasmb 74 ± 2 [3.2] 77 ± 2 [3.2] 76 ± 8 [3.2] 70 ± 4 [3.0]
   Total cortexb 26 ± 0.8 [4.6] 23 ± 0.5 [4.0] 24 ± 1 [4.4] 30 ± 0.1 [4.6]
   Furrow cortexb - - 13 ± 1 [7.6] 16 ± 1 [18]
Minimal force requirements, nNc - - 7.2 2.7
   % of total myosin-II required for this amount of forced - - 12 4.5
  1. a These quantities were calculated based upon the geometry and dimensions of the cell. b These quantities were calculated from the empirically determined concentrations derived from the ratio imaging analysis. c These quantities were calculated based upon the dimensions of the cell. d These quantities were calculate by considering the amount of minimal required force, the physical properties of myosin-II and the amount of myosin-II in the cell.