Lipopolysaccharide enhances the cytotoxicity of 2-chloroethyl ethyl sulfide

Table 3 PMA Enhances the Cytotoxicity of CEES to RAW264.7 Macrophages (24 hours)¹

Assay²	Control	LPS	PMA	CEES	LPS+CEES	PMA+CEES
PI (%)	0.0 ± 0.0 ^a	2.6 ± 1.03^a	1.8 ± 0.96^a	18.7 ± 6.23^b	59.1 ± 6.63^c	41.4 ± 5.0 ^d
MTT³	1.50 ± 0.03^a	1.56 ± 0.06^a	1.71 ± 0.03^a	1.46 ± 0.10 ^b	1.07 ± 0.11^c	1.36 ± 0.04 ^b

¹LPS was used at 25 ng/ml, PMA at 50 ng/ml and CEES 500 μM. Values are means ± SD and means (in a given row) not sharing the same superscript letter are significantly different (p < 0.05). ²PI(%) is the percent of propidium iodide fluorescence over that of the control cells. ³The MTT levels are given as 575 nm absorbance units.

ISSN: 2661-8850