Figure 5
From: Telomere and ribosomal DNA repeats are chromosomal targets of the bloom syndrome DNA helicase
Helicase activity of the GFP-BLM deletion proteins. A. Autoradiogram of the unwinding assay gel. The 0 lane contains no enzyme. The Δlane reaction was heated at 95°C for 5 minutes. The C2 reaction contains 1 ng protein and other reactions with the deletion proteins contain 25 ng. The normal BLM reactions contain the amounts shown in the right graph in panel B. Activities are reported relative to 8 units/ng DNA-dependent ATPase activity and 7 units/ng unwinding activity for normal GFP-BLM. B. Graphic representation of helicase activities relative to normal GFP-BLM. Assays were performed as described previously [4]. Quantitation of the autoradiogram using a phosphorimager is shown in the left and right graphs. Relative DNA-dependent ATPase activities are shown in the left graph. The thin layer ATPase assays are not shown.