Figure 1
From: Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology
RT-PCR analysis of mRNAs in the cytosolic and cytoskeleton-associated fractions. Semi-quantitative RT-PCR of transcripts identified as cytoskeleton-associated by microarray hybridization. Serially diluted cDNA (1:3, 1:9, 1:27, 1:81, 1:243, 1:729 in ddH2O) from the cytosolic (left) and cytoskeleton-associated (right) fractions served as the template for PCR amplification. Other mRNAs that were confirmed using this approach include rps13, thymosin-β4, and gluthathione peroxidase.