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Table 2 Effect of Bax genotype on properties of myofibers in vivo.

From: Immortalization of mouse myogenic cells can occur without loss of p16INK4a, p19ARF, or p53 and is accelerated by inactivation of Bax

Property

Muscle

Bax-positive ave ± SE (n)

Bax-null ave ± SE (n)

Significance*

Cross-sectional area (μm2) of fast‡ myofibers

Soleus

1045 ± 17.0 (451)

785 ± 11.9 (500)

P < 0.0001

 

Diaphragm

873 ± 7.7 (1364)

717 ± 6.9 (1570)

P < 0.0001

Cross-sectional area (μm2) of slow‡ myofibers

Soleus

1399 ± 33.7 (193)

1096 ± 16.6 (270)

P < 0.0001

 

Diaphragm

640 ± 15.0 (136)

547 ± 12.8 (150)

P < 0.0001

Percentage of fast myofibers

Soleus

70.2 ± 1.8 (3)

70.8 ± 0.1 (3)

P > 0.7

 

Diaphragm

90.7 ± 0.7 (5)

91.2 ± 0.8 (5)

P > 0.7

Cross-sectional area (mm2) of whole muscle

Soleus

1.54 ± 0.12 (4)

1.18 ± 0.06 (5)

P = 0.024

Total number of myofibers

Soleus

1081 ± 36 (4)

1102 ± 17 (5)

P > 0.5

  1. ‡Fast and slow myofibers were identified by ATPase staining as described in Methods. *Comparison of Bax-positive to Bax-null with unpaired, two-tailed, t-test.