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Figure 1 | BMC Cell Biology

Figure 1

From: Down-regulation of the M6P/IGF-II receptor increases cell proliferation and reduces apoptosis in neonatal rat cardiac myocytes

Figure 1

Hammerhead ribozyme structure and target sequence of the M6P/IGF2R mRNA (A) and ribozyme activity against M6P/IGF2R RNA in vitro (B). 32P-labeled RNA containing the M6P/IGF2R target sequence was incubated with the hammerhead ribozyme and the reaction products were separated by polyacrylamide gel. The ribozyme cleaves the M6P/IGF2R target efficiently and specifically in vitro. Lane 1: Specific target alone; Lane 2: Specific target incubation with ribozyme at time 0; Lane 3–10: Specific target incubation with ribozyme at a time-course of 5, 10, 20, 40, 80, 160, 320 and 640 minutes; Lane11: Unmatched target alone; Lane 12: Unmatched target incubation with ribozyme at 640 minutes.

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