Figure 11
From: Polyphosphoinositides-dependent regulation of the osteoclast actin cytoskeleton and bone resorption
Transduction of PBD containing gelsolin peptides reduces the association of phosphoinositides WASP and Ezrin proteins Panel A: Western analysis with an antibody to PtdIns P2. Osteoclasts were treated as indicated in the figure. The lysates were immunoprecipitated with either ezrin (lanes 1–3) or WASP (lanes 5–7). Immunoprecipitation with non-immune serum is shown in lanes 4 and 8. Arrows point to IgG HC as well as PtdIns P2 (PIP2) associated ezrin and WASP proteins. Panel B: Western analysis with an antibody to ezrin or WASP. Arrows indicate ezrin and WASP proteins. The results represent one of three experiments performed.