Figure 12
From: Polyphosphoinositides-dependent regulation of the osteoclast actin cytoskeleton and bone resorption
Transduction of PBD containing gelsolin peptides reduces the interaction of WASP with Arp3 protein Osteoclasts were transduced with indicated TAT-proteins and treated with OPN (denoted as (+)) or PBS (denoted as (-)). Equal amount of lysates were immunoprecipitated with anti-Arp3 antibody (lanes 2–9) and non-immune serum (NI, lane 1). The immunoprecipitates were subjected to SDS-PAGE. Panel A. Western analysis with a WASP antibody. Arrow points to WASP protein. Panel B. Western analysis with an Arp3 antibody. The immunoblot shown in panel A was stripped and blotted with an Arp 3 antibody to detect the levels of Arp 3 in each immunoprecipitates. The results represent one of three experiments performed.