Figure 3
From: Polyphosphoinositides-dependent regulation of the osteoclast actin cytoskeleton and bone resorption
The effects of OPN or TAT-HA-protein transduction on PtdIns P 2 association with the transduced peptides Osteoclasts were transduced with the indicated gelsolin peptides and treated with PBS (-) or OPN (+) as shown below the Figure. Lysates were immunoprecipitated with an antibody to HA (lanes 1, 2, 4–11) or non-immune serum (NI; lanes 3 & 12). The immunoprecipitates were subjected to 8% SDS-PAGE (lanes 1 and 2) or 15% SDS-PAGE (lanes 3–12). Panel A: Western analysis with an antibody to PtdIns P2. Panel B: Western analysis with an antibody to HA. The immunoblot shown in panel A was stripped and blotted with a HA-antibody to detect the levels of transduced proteins immunoprecipitated in each lane. Arrowheads point to transduced proteins associated with PtdIns P2. Arrows indicate IgG heavy chain (IgG HC). The results represent one of three experiments performed from three separate osteoclast preparations.