Figure 6
From: Polyphosphoinositides-dependent regulation of the osteoclast actin cytoskeleton and bone resorption
The effects of TAT-HA proteins transduction on actin distribution Confocal microscopy images of mouse osteoclasts stained for actin (red) and HA-protein (green) are shown. An actin enriched patch marked by a white square in Fig. H is magnified in Fig. I. Yellow color indicates the colocalization of transduced peptides with actin. Immunostaining with a non-immune (NI) serum is shown in Panel J. Scale bars- 25 μm (Panels A-H and J) and 5 μm (Panel. I). Arrowheads indicate patches enriched in transduced peptides and actin (Panels C, D, F, G, and H). Arrows indicate typical podosome structures in panels A, B, and E. The results shown are representative of three independent osteoclast preparations and experiments.