Figure 10

Effects of SB203580 on TNF-α priming. Neutrophils were primed by TNF-α (25 ng/ml, 20 min, 37°C) in the absence or presence of the p38 MAPK inhibitor SB203580 (1 μM). (A) The cells were stimulated with cytochalasin B (5 μg/ml) and the extracellular production of superoxide anion was measured by CL. The effect of SB203580 on PMA-induced superoxide anion production was used as control. (B) Neutrophils were incubated with TNF-α (25 ng/ml) in the absence or presence of SB203580 (1 μM)), or with SB203580 alone (20 min, 37°C) and were paraformaldehyde-fixed, incubated with phycoerythrin-conjugated anti-CR3 antibodies (CD18/CD11b), analysed by flow cytometry and compared with control cells. Calculation were made from the mean fluorescence intensity of each cell population and expressed as percentage of the value obtained in control cells. The results are given as mean ± SE of three independent experiments.