Figure 2
From: Cytochalasin B triggers a novel pertussis toxin sensitive pathway in TNF-alpha primed neutrophils
fMLF stimulation of TNF-α primed neutrophils. Neutrophils were pre-incubated in the absence (dashed line) or presence (solid line) of TNF-α (25 ng/ml) for 20 min at 37°C after which they were stimulated with fMLF. The extracellular production of superoxide anion after addition of the peptide (10-7M) was measured by isoluminol-amplified CL. Responses of CL are given as Mcpm (106 counts per minutes). The figure shows the kinetics of representative experiments.