Figure 3
From: Cytochalasin B triggers a novel pertussis toxin sensitive pathway in TNF-alpha primed neutrophils
Time dependency of the TNF-α effect Responses to cytochalasin B after incubation with TNF-α for different periods of time. Neutrophils were pre-incubated with TNF-α (25 ng/ml, 37°C) under various time-periods (5–40 min), before they were stimulated with cytochalasin B. The production of superoxide anions was measured by isoluminol-amplified CL as described above.