Figure 4

Pertussis toxin (PtX) sensitivity of cytochalasin B induced superoxide anion production and CR3 upregulation in TNF-α primed neutrophils. Neutrophils were preincubated with TNF-α (25 ng/ml, 20 min, 37°C) before they were incubated with PtX (500 ng/ml) for various time-periods (20–140 min). The cells were either paraformaldehyde-fixed and analyzed by flow cytometry for CR3 upregulation (closed squares), or stimulated with cytochalasin B, and the superoxide anion production were measured by CL (open squares). All analyzed populations of PtX incubated cells were compared to TNF-α primed cells not exposed to PtX.