Figure 5
From: Mutations in the IGF-II pathway that confer resistance to lytic reovirus infection
IGF-II modulates reovirus resistance in RIE-1 and 6B72 cells. RIE-1 and 6B72 cells expressing either the IGF-II pro-hormone (proIGF2) or the alternatively spliced transcript (IGF2SV, Figure 3D) were challenged with serial dilutions of reovirus type 1 (upper panel), and the surviving cells were stained with gentian violet 4 days post-infection. The multiplicity of infection (MOI) for each row is indicated. pro-IGF-II converted RIE-I cells to a reovirus resistant phenotype (RIE-1/proIGF2) but had little if any effect on already-resistant 6B72 cells (6B72/proIGF2). Plasmids expressing the alternatively spliced Igf2 transcript had no effect on RIE-1 cells (RIE-1/IGF2SV) but abrogated virus resistance in 6B72 cells (6B72/IGF2SV). The experiment was repeated 3 times. Expression of Igf2 transgenes (lower panel) was monitored by Northern blot hybridization, and the expression of Igf2 in RIE-1 cells is shown for comparison. Expression of Igf2 in 6B72 is not shown.