Figure 7From: 4-D single particle tracking of synthetic and proteinaceous microspheres reveals preferential movement of nuclear particles along chromatin – poor tracksChromatin intensity analysis showing the preprocessing steps for the Hoechst 33342 images: a) unprocessed original image; b) Gaussian smoothing; c) image classified into 8 regions of grey values; d) localization of particles in the cell nucleus (red); e) measuring the mean grey value intensity around the center of mass for each individual vimentin particle (see Methods for a complete description).Back to article page