Figure 6
Effect of PE on the expression of cell cycle regulators, p27kip1, p21ip1/waf1 and pRB. Preconfluent, serum-deprived cells were incubated with PE (5 μM) or vehicle for up to 48 h. Expression of cell cycle regulators was determined by Western blot analysis of whole cell lysates as described in Methods. The panels are representative Western blots showing the effect of PE on the expression of p27kip1 (A), p21cip1/waf1 (B), pRb (C), and lamin A/C (D) protein levels respectively. Percentages representing the quantitation of protein bands by densitometric analysis of blots obtained from three different experiments are indicated on top of each immunoblot.