Figure 5
From: Regulation of cell cycle by the anaphase spindle midzone
Normal interphase progression following removal of the midbody. A cell was cut at the end of cytokinesis to form daughter cells with or without the midbody (A, b, arrow). Subsequent long-term time-lapse imaging indicated a normal phase morphology for both cells (A). The cell with midbody entered mitosis 10 h 40 min after cutting, whereas the cell without midbody entered mitosis ~1 h afterwards (e, f, large arrows). Fluorescence imaging of aurora-B-GFP, a midbody component, confirmed that the midbody is completely segregated into one of the daughter cells (B; cutting indicated by dotted lines) Bar, 20 μm.