Figure 3
From: Fas induces apoptosis in human coronary artery endothelial cells in vitro
Induction of apoptosis in cultured human coronary artery endothelial cells by activation of Fas. Human coronary artery endothelial cells (HCAEC) were incubated with purified recombinant human Fas ligand (FasL), Fas-activating monoclonal antibody CH-11 (Fas mAB), or nonspecific isotype-matched immunoglobulins (N.S.IgM) at the indicated concentrations (ng/ml) in serum-free medium. After 20 hours, apoptosis was detected as described in Figure 1. Bars are the mean + S.E.M. of at least three determinations; ** = p < 0.01 versus control (CTL) and * = p < 0.05 versus CTL by ANOVA and Student-Newman-Keul's test.