Figure 5

Fluorescence microscopy of Bacillus subtilis cells expressing GFP-MreB from an ectopic site on the chromosome. A) wild type cells (helical filaments), B) 2 or C) 4 doubling times after depletion of MreC (loss of filaments), D) 2 or E) 6 doubling times after depletion of Mbl (abnormal filaments and later loss of filaments), F) 2 or G) 6 doubling times after depletion of MreBH (abnormal filaments). Grey arrows point out extended GFP-MreB filaments, and the white arrow indicates GFP-MreB foci. Grey bars 2 μm.