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Table 1 Interaction of Iporin ΔN847 with different rab proteins/isoforms

From: Identification and characterization of Iporin as a novel interaction partner for rab1

 

prey Iporin ΔN847

bait

control

his3

lacZ

pAS2-1

+++

-

-

rab1a wt

+++

+

+/-

rab1a S25N

+++

-

-

rab1a Q70L

+++

+++

+++

rab1b wt

+++

+/-

-

rab1b S22N

+++

-

-

rab1b Q67R

+++

++

+++

rab1b Q67L

+++

++

+++

rab2 wt

+++

-

-

rab2 S20N

+++

-

-

rab2 Q65L

+++

-

-

rab6 wt

+++

-

-

rab6 T27N

+++

-

-

rab6 Q72R

+++

-

-

Ypt1 wt

+++

++

++

Ypt1 S22N

+++

-

-

Ypt1 Q67L

+++

+++

+++

  1. Results obtained from co-transformation assays in yeast. Clones were cultivated on selection plates lacking tryptophan and leucine (control) or lacking additionally histidine and were supplemented with 50 mM 3-aminotriazol (his3). The β-galactosidase activity was determined using X-gal as substrate on filter lift assays (lacZ). - = no growth on selection plates or no β-galactosidase activity; +/- = background growth or β-galactosidase activity appears overnight; + = slow growth; ++ = strong growth or β-galactosidase activity appears after a few hours; +++ = very fast growth or high β-galactosidase activity.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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