Figure 6
From: Trapping of normal EB1 ligands in aggresomes formed by an EB1 deletion mutant
APC is present in EB1-ΔN2-GFP aggresomes. Panels A-C. Cells expressing EB1-ΔN2-GFP were fixed and co-immunostained for GFP (green, panel A) and APC (red, panel B). A merged image is shown in panel C. APC is seen in aggresomes in transfected cells and at peripheral sites in adjacent untransfected cells. Bar = 10 μm. Panels D-F. A single 0.365 μm confocal section through a cell expressing EB1-ΔN2-GFP. APC immunoreactivity (panel E) in aggresomes is heterogeneous but only seen in association with GFP immunoreactivity (panels D and F). Bar = 5 μm. Panels G-I. Cells expressing EB1-ΔN2-GFP were fixed and co-immunostained for GFP (green, panel G) and EB3 (red, panel H). EB3 is seen at microtubule tips but is not present in EB1-ΔN2-GFP aggresomes. Bar = 20 μm. Images in panels A-C and G-I are projections of confocal image stacks.