Figure 6

Effects of AP-9 on MMPs release and activation in undifferentiated and differentiated THP-1 cells. (a) Image of gelatin zymography. Top two bands correspond to MMP-9 gelatinase, lower two bands to MMP-2 gelatinase. (b), (c), (d), (e) Densitometry analysis of MMP activity. The value of MMP activity of THP-1 cells without AP-9 is shown as control (100%). Mean ± SE of at least 6 independent samples per group. p < 0.05, Student's t test. (b) Effects of AP-9 on pro-MMP-9 activity of undifferentiated THP-1 cells. (c) Effects of AP-9 on pro-MMP-9 activity of differentiated THP-1 cells. (d) Effects of AP-9 on MMP-9 activity of differentiated THP-1 cells. (e) Effects of AP-9 on MMP-2 activity of differentiated THP-1 cells.